This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison.
Homologs of human HEXA gene
Homologene was used to determine the gene homologs of Beta-hexosaminidase subunit alpha (HEXA) found in human.
Eight species were found to contain a gene homolog with humans.
The document below contains DNA sequences (in FASTA format) of human HEXA and its putative homologs, which have been limited to the first ~1100 nucleotides after eliminating the long sequences of unknown nucleotides.
Eight species were found to contain a gene homolog with humans.
The document below contains DNA sequences (in FASTA format) of human HEXA and its putative homologs, which have been limited to the first ~1100 nucleotides after eliminating the long sequences of unknown nucleotides.
homologs_gene_sequences.doc | |
File Size: | 37 kb |
File Type: | doc |
List of HEXA gene homologs
Alignments
Alignments were produced using the protein sequences found in Homologene. ClustalW2 and T-Coffee each produced a valid alignment.
Multiple gene sequence alignment using ClustalW2
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Analysis
Since the gene sequences for human and the 8 homologs are too long to be processed by most of the databases, I had to truncate the number of nucleotides in each species to around 1100 nucleotides. I had no trouble aligning the gene sequences using T-Coffee 9.01. The results from T-Coffee suggested that the gene alignment are bad and most of the sequences are not well-conserved. This is because I truncated the sequences to only 1100 nucleotides. This might have biased the results. On the other hand, ClustralW yielded the almost same result as T-Coffee. Hence, protein analysis is a better way to show the Hexa homology.